Saranya Krishnan

Hi, I am Saranya. I hold MS Forensic Science (Biology track) from International Forensic Research Institute (IFRI),Florida International University, Miami and MSc Biotechnology from Bharatiar University, India. During my graduate degree in US, myresearch work was in the Genomic Analyses of pathogenic and Non pathogenic Fungi isolated from ambrosia beetles in avocado trees using Fragment analysis and Multi locus sequence typing. I am a person passionate in Forensic Biology, especially the role of DNA in Forensics.

Sanger Sequencing

Sequencing is a process of determining the precise order of nucleotides in a DNA template after amplification using specific loci. Sanger sequencing was invented by Fredrick Sanger in 1977.It is a widely used sequencing method and now replaced by Next-Generation sequencing methods. In Sanger sequencing, only¬† fragments of DNA (specific locus) can be amplified and …

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Length Heterogeneity Polymerase Chain Reaction

Length Heterogeneity Polymerase Chain Reaction   Length Heterogeneity Polymerase Chain Reaction (LH-PCR) reveals the length heterogeneity of amplicons. The procedure indicates the natural variations of insertion and deletion of nucleotides. LH PCR exposes the allelic profile pattern of DNA in a particular loci. Species-specific differentiation is possible using this method. The procedure is rapid simple …

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