Sequencing is a process of determining the precise order of nucleotides in a DNA template after amplification using specific loci. Sanger sequencing was invented by Fredrick Sanger in 1977.It is a widely used sequencing method and now replaced by Next-Generation sequencing methods. In Sanger sequencing, only fragments of DNA (specific locus) can be amplified and …
Length Heterogeneity Polymerase Chain Reaction Length Heterogeneity Polymerase Chain Reaction (LH-PCR) reveals the length heterogeneity of amplicons. The procedure indicates the natural variations of insertion and deletion of nucleotides. LH PCR exposes the allelic profile pattern of DNA in a particular loci. Species-specific differentiation is possible using this method. The procedure is rapid simple …
Polymerase Chain Reaction (PCR) is the process of amplification of fragments of DNA for further analysis in biomedical research and in Forensics. PCR was invented by Karry Mullis in 1983.After the extraction of DNA, DNA is amplified using PCR. There are three different steps in PCR- Denaturation, Annealing and Extension. Amplification process takes place inside …