Mitochondria is a double-layered membrane-bound organelle found in eukaryotic organisms. The organelle is also known as a powerhouse since it generates most of the cell’s adenosine triphosphate supply as an energy source. DNA evidence samples that might be too little or degraded for Nuclear DNA analysis or miniSTR analysis. Mitochondrial DNA is inherited from one …
Alcohol analysis and Forensic issues Ethyl alcohol (Ethanol ) is the commonly used oldest drug consumed by a human for many years. Ethanol is a volatile liquid soluble in water and has a characteristic taste. Alcohols are a group of organic chemicals and have a hydroxyl group attached to the hydrocarbon chain of the molecule. …
Forensic and Clinical issues in Alcohol analysis Read More »
MARYLAND, Petitioner v. Alonzo Jay KING, Jr. FORENSIC CASE In 2003, a man broke into a woman’s house with his face concealed, armed with a gun, and raped her. The police were unable to find the victim but were able to get the assailant’s DNA from the victim. Later in 2009, Alonzo king was …
Monica Lewinsky blue dress In 1988, independent counsel Kenneth Star was investigating allegations that then the US president William Jefferson Clinton had a sexual relationship with white house intern Monica Lewinsky. President Clinton denied the allegations publicly since there was no evidence at that time. Monica Lewinsky blue dress was bought into FBI Laboratory …
Blue Dress of Lewinsky and Forensic Method to Detect Semen Stain Read More »
Forensic DNA Testing evolved during the past few years from single-locus Restriction Fragment Length Polymorphism (RFLP) reaction to polymerase chain reaction (PCR). The processes reduced the time frame which was 6 to 8 weeks to a few hours now. There are various DNA markers and they have been divided into four different quadrants depending upon the power …
Forensic Biology has been expanded today with various molecular biology discoveries and technological advances. Numerous methods adopted by forensic laboratories include Polymerase Chain Reaction, Fragment Analysis, Software systems, and automatic liquid handling methods. Progression of Sanger sequencing, Next Generation sequencing, Mitochondrial analysis is the widely accepted technologies and tools for solving criminal cases. Beyond Fingerprint …
Phenol Chloroform Extraction is a method used for extracting DNA from blood stains and liquid blood. Bloodstains or liquid blood are placed in a centrifuge tube. The stain extraction buffer (salts, detergents, buffer,10mm tris,100 mm NaCl, 39Mm Dithioreitol (DTT) ,10 Mm EDTA, 2% SDS (sodium dodecyl sulfate) is added. Salts and detergents help solubilize cell …
Human DNA has a very significant role in crime scene investigation by bringing the culprit in front of justice and in exonerating the innocent. Domestic animals such as cats and dogs living in human habitat deposit hairs can aid in finding the suspect related to a crime. The botanical specimen found in the victim’s body …
STR (Short tandem repeat) genotyping is a forensic investigative tool used to identify alleles at specific loci between questioned and reference samples. STR genotyping is a method that employs polymerase chain reaction (PCR) instead of Restriction Fragment Length Polymorphism(RFLP).RFLP method uses restriction enzymes to cut the DNA.STR amplicons are separated and detected using Capillary Electrophoresis. …
Colin Pitchfork was the first convicted murderer and rapist found using DNA typing. He was caught as a result of mass DNA screening. Pitchfork lived in a village in the county of Leicestershire, England. He was working in Hampshires Bakery until he was arrested for the crimes. In 1983, Lynda Mann was sexually assaulted and …