FTA Paper was developed by Lee Burgoyne at Flinders University in Australia and is a method of storing DNA. It is an absorbent cellulose-based paper containing chemical substances to protect the DNA from nuclease degradation and preserve the paper and DNA from bacterial growth. DNA on FTA paper is stable at room temperature over a …
Chelex is a cation exchange resin ( styrene-divinylbenzene copolymer containing paired iminodiacetate ions) used for PCR analysis. Resin is regenerated in dilute acid and operates in a solution of PH 4 or higher. A 5 % suspension should have a PH between 10 and 11. The resin strongly chelate metals and remove heme inhibitors and …
DNA extraction/isolation is the purification of DNA using combination of various methods. DNA Extraction is a significant procedure in molecular biological methods and in forensic analysis. Several different extraction kits and protocols/methods are available. Proper extraction methods and procedures should be chosen to get the process optimized and also to save time. The goal …
Test For Body Fluids 25 % of sexual assaults happen in children under the age of 16 and the perpetrator in most cases will be a family member. In such situations, it is not DNA that is under dispute but the type of body fluids. There are different procedures and methods to analyze and identify …
Saliva is an extracellular fluid secreted by salivary glands in the mouth. Saliva in humans, consists of water, electrolytes, mucus, white blood cells, epithelial cells, amylase, lipase, IgA, and lysozymes. Alpha amylase is present in humans, elephants, rats, and pigs. Amylase in humans is 42-59 U/ml. STARCH IODINE PRESUMPTIVE TEST Enzyme amylase is a component …
Semen analysis Semen, also known as Spermatozoa is a type of body fluid that is secreted by sexual organs of the male reproductive system to fertilize female ova. In certain cases to analyze rape or sexual assaults, a presumptive and confirmatory test of semen(semen analysis) is performed. Screening test A screening test is performed to …
What is Forensic Serology? Forensic serologist analyze semen, feces, urine, saliva etc. Serology is the identification of blood and blood proteins. Serology is described as a range of laboratory tests utilizing antigen and serum antibody reactions. There are two different types of blood/body fluids transfer. Direct transfer and indirect transfer. Current serological tests analyses the …
Sequencing is a process of determining the precise order of nucleotides in a DNA template after amplification using specific loci. Sanger sequencing was invented by Fredrick Sanger in 1977.It is a widely used sequencing method and now replaced by Next-Generation sequencing methods. In Sanger sequencing, only fragments of DNA (specific locus) can be amplified and …
Length Heterogeneity Polymerase Chain Reaction Length Heterogeneity Polymerase Chain Reaction (LH-PCR) reveals the length heterogeneity of amplicons. The procedure indicates the natural variations of insertion and deletion of nucleotides. LH PCR exposes the allelic profile pattern of DNA in a particular loci. Species-specific differentiation is possible using this method. The procedure is rapid simple …
Polymerase Chain Reaction (PCR) is the process of amplification of fragments of DNA for further analysis in biomedical research and in Forensics. PCR was invented by Karry Mullis in 1983.After the extraction of DNA, DNA is amplified using PCR. There are three different steps in PCR- Denaturation, Annealing and Extension. Amplification process takes place inside …