Forensics

Sanger Sequencing

Sequencing is a process of determining the precise order of nucleotides in a DNA template after amplification using specific loci. Sanger sequencing was invented by Fredrick Sanger in 1977.It is a widely used sequencing method and now replaced by Next-Generation sequencing methods. In Sanger sequencing, only¬† fragments of DNA (specific locus) can be amplified and …

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Length Heterogeneity Polymerase Chain Reaction

Length Heterogeneity Polymerase Chain Reaction   Length Heterogeneity Polymerase Chain Reaction (LH-PCR) reveals the length heterogeneity of amplicons. The procedure indicates the natural variations of insertion and deletion of nucleotides. LH PCR exposes the allelic profile pattern of DNA in a particular loci. Species-specific differentiation is possible using this method. The procedure is rapid simple …

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