Laser Microdissection (LMD) is a new technique of separating specific cells or tissue from a mixture. This procedure can isolate cells by cutting away unwanted cells or tissues using a microscopic system equipped with a laser. .LMD technique does not change the morphology/chemistry of the cells. The major issue in forensics is the less amount …
DNA IQ system uses a unique approach for DNA isolation. In this method, paramagnetic resin is used in this procedure. The resin has a definite DNA binding capacity and will only bind to a specific, certain amount of DNA. DNA IQ system avoids the use of organic solvents such as phenol and eliminates multiple centrifugation …
Differential Extraction The Standard differential extraction (differential lysis) method was devised by Gill, Jeffreys, and Werrett in Nature,1985. It is a process used to separate male and female DNA from a sexual assault sample evidence. Differential extractions can be performed from both vaginal swabs and semen stains. The successful separation of cells in this extraction …
FTA Paper was developed by Lee Burgoyne at Flinders University in Australia and is a method of storing DNA. It is an absorbent cellulose-based paper containing chemical substances to protect the DNA from nuclease degradation and preserve the paper and DNA from bacterial growth. DNA on FTA paper is stable at room temperature over a …
Chelex is a cation exchange resin ( styrene-divinylbenzene copolymer containing paired iminodiacetate ions) used for PCR analysis. Resin is regenerated in dilute acid and operates in a solution of PH 4 or higher. A 5 % suspension should have a PH between 10 and 11. The resin strongly chelate metals and remove heme inhibitors and …
DNA extraction/isolation is the purification of DNA using combination of various methods. DNA Extraction is a significant procedure in molecular biological methods and in forensic analysis. Several different extraction kits and protocols/methods are available. Proper extraction methods and procedures should be chosen to get the process optimized and also to save time. The goal …
Test For Body Fluids 25 % of sexual assaults happen in children under the age of 16 and the perpetrator in most cases will be a family member. In such situations, it is not DNA that is under dispute but the type of body fluids. There are different procedures and methods to analyze and identify …
Saliva is an extracellular fluid secreted by salivary glands in the mouth. Saliva in humans, consists of water, electrolytes, mucus, white blood cells, epithelial cells, amylase, lipase, IgA, and lysozymes. Alpha amylase is present in humans, elephants, rats, and pigs. Amylase in humans is 42-59 U/ml. STARCH IODINE PRESUMPTIVE TEST Enzyme amylase is a component …