Month: May 2021

Terminal Restriction Fragment Length Polymorphism

T-RFLP (Terminal Restriction fragment Length Polymorphism) is one of the most common DNA fingerprinting techniques in forensic applications. T-RFLP is a microbial profiling method and is an amplification method for amplifying the 16S rRNA genes. TRFLP is an amplification method using fluorescent-labeled end primer. Amplicons are digested using restriction endonucleases. Fragments (different sizes) are separated …

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Denaturing Gradient Gel Electrophoresis

Denaturing Gradient Gel Electrophoresis involves PCR amplification of 16srRNA gene sequence. It involves the separation of amplicons of the same length but with different basepair sequences on polyacrylamide gel containing DNA denaturant. Few steps in Denaturing Gradient Gel Electrophoresis steps are as follows DNA Extraction from Environmental samples. Amplification of DNA Fragments by PCR Incomplete …

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Single stranded Conformation Polymorphism

Single-stranded Conformation Polymorphism (SSCP)¬†distinguishes DNA molecules even they are of the same size due to the differences in the nucleotide sequences. Small changes in the nucleotide sequences can be detected through conformational changes in the DNA. SSCP analysis includes the extraction of DNA from environmental samples. PCR is then conducted using fluorescent-labeled forward primer and …

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Identification of Human remains using Rapid DNA Analysis

DNA Genotyping is a laboratory method for the identification of victims from a disaster or mass casualty events. The DNA Commission of the International Society for Forensic Genetics (ISFG) provided laboratory guidelines for sample collection, storage, DNA purification, and Genotyping. Software and bioinformatics tools that have been developed. Human Remains cannot be recovered from the …

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